prorenin  (Innovative Research Inc)

Journal: American journal of physiology. Renal physiology

Article Title: Renal tubular (pro)renin receptor deletion exacerbates kidney injury in db/db mice

doi: 10.1152/ajprenal.00231.2025

Figure Lengend Snippet: Plasma prorenin/renin concentration (A), plasma sPRR levels (B) urine prorenin/renin/creatinine ratio at 30 weeks (C), kidney renin mRNA expression (D), kidney PRR mRNA expression by RT-PCR (E), kidney cortical protein expression by immunoblotting (F) and quantification (G) in male non-diabetic control, db/db, PRR KO db/db and PRR KO mice (N=5–7/group). **** p<0.0001, *** p<0.001, ** p<0.01 * p<0.05 using two-way ANOVA with Tukey post-hoc test.

Article Snippet: Specific EIA kits were used to measure plasma and urine total prorenin/renin content which is the total amount of immunoreactive prorenin and renin (Innovative Research, Novi, MI, SKU: IMSPRENKTT) and plasma sPRR (IBL America, Minneapolis, MN, SKU: IB59131).

Techniques: Clinical Proteomics, Concentration Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

Journal: Acta physiologica (Oxford, England)

Article Title: Collecting duct Renin Regulates Potassium Homeostasis in Mice

doi: 10.1111/apha.13899

Figure Lengend Snippet: Analysis of electrolyte balance in NK and HK - treated floxed and CD renin KO mice. (a) 24-h urinary Na+, K+, and Cl− excretion. (b) Na+, K+, and Cl− balance. (c) Plasma Na+, K+, and Cl− concentration. N=10 per group. Data are mean ± SEM. *p<0.05, †p<0.01, ‡p<0.001.

Article Snippet: The values were expressed as nanograms per milliliter per hour of generated Ang I. Additionally, total renin/prorenin released into the plasma and urine were measured by ELISA assay using a Total mouse renin/prorenin ELISA kit (MPRENKT-TOT, Molecular Innovations, Novi, USA) according to the manufacturer’s instructions.

Techniques: Concentration Assay

Journal: Acta physiologica (Oxford, England)

Article Title: Collecting duct Renin Regulates Potassium Homeostasis in Mice

doi: 10.1111/apha.13899

Figure Lengend Snippet: Renin expression in the kidney in NK and HK - treated floxed and CD renin KO mice. (a, b) RT-qPCR analysis of renin mRNA in the renal cortex (a) and medulla (b), expression was normalized by GAPDH. N = 5–6 per group. Data are mean ± SEM. *p<0.05, †p<0.01, ‡p<0.001. (c, d) Representative immunoblotting and densitometric analysis of prorenin expression in the renal cortex (c) and renal medulla (d), the expression was normalized by β-actin. N=3 in Floxed+NK and CD renin KO+NK group, N=4 in Floxed+HK and CD renin KO+HK group. Data are mean ± SEM. *p<0.05, ‡p<0.001 vs. NK; †p<0.01 vs. Floxed.

Article Snippet: The values were expressed as nanograms per milliliter per hour of generated Ang I. Additionally, total renin/prorenin released into the plasma and urine were measured by ELISA assay using a Total mouse renin/prorenin ELISA kit (MPRENKT-TOT, Molecular Innovations, Novi, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Acta physiologica (Oxford, England)

Article Title: Collecting duct Renin Regulates Potassium Homeostasis in Mice

doi: 10.1111/apha.13899

Figure Lengend Snippet: Urinary and plasma renin levels in NK and HK - treated floxed and CD renin KO mice. (a, b) The urine samples (a) and plasma samples (b) were assayed for renin activity, total renin content, active renin content, and prorenin content. (c, d) The urine samples (c) and plasma samples (d) were assayed for the total renin/prorenin by ELISA, the ELISA kit does not differentiate between prorenin and renin. N=10 per group. Data are mean ± SEM. *p<0.05, †p<0.01, ‡p<0.001. ICV: interassay coefficient of variation.

Article Snippet: The values were expressed as nanograms per milliliter per hour of generated Ang I. Additionally, total renin/prorenin released into the plasma and urine were measured by ELISA assay using a Total mouse renin/prorenin ELISA kit (MPRENKT-TOT, Molecular Innovations, Novi, USA) according to the manufacturer’s instructions.

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay

Journal: Acta physiologica (Oxford, England)

Article Title: Collecting duct Renin Regulates Potassium Homeostasis in Mice

doi: 10.1111/apha.13899

Figure Lengend Snippet: Responses in systemic and intrarenal aldosterone to NK and HK intake in floxed and CD renin KO mice. (a-c) Aldosterone levels in the urine (a), renal cortex and medulla (b), plasma and adrenal gland (c) were determined by ELISA. N=6–18 per group. Data are mean ± SEM. *p<0.05, †p<0.01, ‡p<0.001.

Article Snippet: The values were expressed as nanograms per milliliter per hour of generated Ang I. Additionally, total renin/prorenin released into the plasma and urine were measured by ELISA assay using a Total mouse renin/prorenin ELISA kit (MPRENKT-TOT, Molecular Innovations, Novi, USA) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Acta physiologica (Oxford, England)

Article Title: Collecting duct Renin Regulates Potassium Homeostasis in Mice

doi: 10.1111/apha.13899

Figure Lengend Snippet: Responses in renal CYP11B2 expression to NK and HK intake in floxed and CD renin KO mice. (a) Representative immunoblotting (Anti-CYP11B2, ab167413, Abcam) and densitometric analysis of CYP11B2 in the renal cortex, medulla, and adrenal gland, expression was normalized by β-actin. N=3 in Floxed+NK and CD renin KO+NK group, N=4 in Floxed+HK and CD renin KO+HK group. Data are mean ± SEM. †p<0.01 vs. NK; *p<0.05, ‡p<0.001 vs. Floxed. (b) RT-qPCR analysis of CYP11B2 mRNA in the renal cortex, medulla, and adrenal gland, expression was normalized by GAPDH. N=5–6 per group. Data are mean ± SEM. *p<0.05, †p<0.01, ‡p<0.001.

Article Snippet: The values were expressed as nanograms per milliliter per hour of generated Ang I. Additionally, total renin/prorenin released into the plasma and urine were measured by ELISA assay using a Total mouse renin/prorenin ELISA kit (MPRENKT-TOT, Molecular Innovations, Novi, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Western Blot, Quantitative RT-PCR

Journal: Acta physiologica (Oxford, England)

Article Title: Collecting duct Renin Regulates Potassium Homeostasis in Mice

doi: 10.1111/apha.13899

Figure Lengend Snippet: Representative immunoblotting (Anti-CYP11B2, MABS1251, Sigma-Aldrich) and densitometric analysis of CYP11B2 in the renal cortex, medulla, and adrenal gland, expression was normalized by β-actin. N=3 in Floxed+NK and CD renin KO+NK group, N=4 in Floxed+HK and CD renin KO+HK group. Data are mean ± SEM. ‡p<0.001 vs. NK; *p<0.05 vs. Floxed.

Article Snippet: The values were expressed as nanograms per milliliter per hour of generated Ang I. Additionally, total renin/prorenin released into the plasma and urine were measured by ELISA assay using a Total mouse renin/prorenin ELISA kit (MPRENKT-TOT, Molecular Innovations, Novi, USA) according to the manufacturer’s instructions.

Techniques: Western Blot, Expressing

Journal: Acta physiologica (Oxford, England)

Article Title: Collecting duct Renin Regulates Potassium Homeostasis in Mice

doi: 10.1111/apha.13899

Figure Lengend Snippet: Immunoblotting and densitometric analysis of NCC, pNCC-T53, α-ENaC, β-ENaC, γ-ENaC, ROMK, α-BK, and α-NKA in the renal cortex in NK and HK - treated floxed and CD renin KO mice. Expression was normalized by β-actin. N=3 in Floxed+NK and CD renin KO+NK group, N=4 in Floxed+HK and CD renin KO+HK group. Data are mean ± SEM. ‡p<0.001 vs. NK; *p<0.05, †p<0.01 vs. Floxed.

Article Snippet: The values were expressed as nanograms per milliliter per hour of generated Ang I. Additionally, total renin/prorenin released into the plasma and urine were measured by ELISA assay using a Total mouse renin/prorenin ELISA kit (MPRENKT-TOT, Molecular Innovations, Novi, USA) according to the manufacturer’s instructions.

Techniques: Western Blot, Expressing

Journal: Acta physiologica (Oxford, England)

Article Title: Collecting duct Renin Regulates Potassium Homeostasis in Mice

doi: 10.1111/apha.13899

Figure Lengend Snippet: Immunoblotting and densitometric analysis of α-ENaC, β-ENaC, γ-ENaC, ROMK, α-BK, and α-NKA in the renal medulla in NK and HK - treated floxed and CD renin KO mice. Expression was normalized by β-actin. N=3 in Floxed+NK and CD renin KO+NK group, N=4 in Floxed+HK and CD renin KO+HK group. Data are mean ± SEM. ‡p<0.001 vs. NK; *p<0.05, †p<0.01 vs. Floxed.

Article Snippet: The values were expressed as nanograms per milliliter per hour of generated Ang I. Additionally, total renin/prorenin released into the plasma and urine were measured by ELISA assay using a Total mouse renin/prorenin ELISA kit (MPRENKT-TOT, Molecular Innovations, Novi, USA) according to the manufacturer’s instructions.

Techniques: Western Blot, Expressing